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KMID : 0385520130260010099
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Analytical Science & Technology
2013 Volume.26 No. 1 p.99 ~ p.105
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Quality inspections of active components in oxidative hair coloring products by UPLC
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Kim Dong-Gyu
Kim Yoo-Kyung
Yun Eun-Sun
Hong Mi-Sun
Shin Ji-Young
Jeong Yoon-Kyung
Kim Jung-Hun
Chae Young-Zoo
Park Seung-Kook
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Abstract
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To identify oxidative hair dyes in hair-coloring products, the thin-layer chromatography (TLC) screening method was used in accordance with Korean Quasi-drug Codex. However, the TLC method is not reliable when there are very small amount of materials to be tested or when Rf values of several components are similar. In this study, Ultra Performance Liquid Chromatography (UPLC) with a rapid sample preparation method was developed for the reliable and sensitive identification of active components contained in oxidative hair-coloring products. Hexane-distilled water was used for the extraction of active components contained in the products prior to UPLC analysis. The limit of detection of active components was 6.7-77.9 μg/L, and the limit of quantitation was 22.3-259.7 μg/L. Except for α-naphthol, the range of recovery ratio was 96.2-101.5%. From this study, we demonstrated that oxidative active hair-coloring components can easily be analyzed by
rapid extraction method followed by UPLC analysis.
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KEYWORD
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oxidative hair dyes, hair coloring products, quality inspection, TLC, UPLC,
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